解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::The cell reproduction cycle of parasitic wall-free bacteria, mycoplasma, is reviewed. DNA replication of Mycoplasma capricolum starts at a fixed site neighboring the dnaA gene and proceeds to both directions after a short arrest in one direction. The initiation frequency fits to the slow speed of replication fork and ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)00209-6
更新日期:1999-08-01 00:00:00
abstract::Identification of proteoglycans in two human malignant mesothelioma cell lines, one with epithelial differentiation and the other with fibroblast-like phenotype, and the effects of epidermal (EGF), insulin-like (IGF-I) and platelet-derived (PDGF-BB) growth factors on the synthesis of hyaluronan (HA) and proteoglycans ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)80131-x
更新日期:1999-07-01 00:00:00
abstract::Cryptogein, a 98 amino acid protein secreted by the fungus Phytophthora cryptogea, induces a hypersensitive response and systemic acquired resistance in tobacco plants (Nicotiana tabacum var Xanthi). The mode of action of cryptogein has been studied using tobacco cell suspensions. The recognition of this elicitor by a...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)80123-0
更新日期:1999-06-01 00:00:00
abstract::The elucidation of the multiple signaling cascades coupled to the TSH receptor has offered new approaches in the understanding of the pathogenesis of Graves' disease. Here we review findings showing that immunoglobulins from Graves' patients are heterogeneous, bind to different epitopes and, similarly to TSH, activate...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)80090-x
更新日期:1999-05-01 00:00:00
abstract::The ability of asialo-thyroglobulin to bind the thyroid RHL-1 subunit of the asialoglycoprotein receptor has been investigated. Ligand blot assays show that the recombinant carbohydrate recognition domain of the thyroid RHL-1 subunit specifically interacts with rat desialated thyroglobulin. Moreover, RT-PCR and Wester...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)80100-x
更新日期:1999-05-01 00:00:00
abstract::Thyroid peroxidase is a member of a family of mammalian peroxidases that includes myeloperoxidase, lactoperoxidase, eosinophil peroxidase, and salivary peroxidase. Protein sequences showing a high degree of sequence similarity with mammalian peroxidases have recently been observed in several invertebrate species. A mu...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)80110-2
更新日期:1999-05-01 00:00:00
abstract::The cholesterol lowering drug lovastatin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, blocks DNA synthesis and proliferation of thyrotropin (TSH) primed FRTL-5 rat thyroid cells. The blockade can be completely prevented and/or reversed by mevalonate and largely prevented and/o...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)80072-8
更新日期:1999-04-01 00:00:00
abstract::The thyroid hormones L-thyroxine and triiodo-L-thyronine have profound effects on postembryonic development of most vertebrates. Analysis of their action in mammals is vitiated by the exposure of the developing foetus to a number of maternal factors which do not allow one to specifically define the role of thyroid hor...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)80082-0
更新日期:1999-04-01 00:00:00
abstract::The regulation of transcription of the pckA gene coding for phosphoenolpyruvate carboxykinase in Escherichia coli was analysed by site-directed mutagenesis of the promoter region and measurement of in vitro transcription initiation. Mutation of the guanine residue at position -14 to either cytosine or thymine was foun...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)80052-2
更新日期:1999-03-01 00:00:00
abstract::The development of in vitro repair assays with human cell-free extracts led to new insights on the mechanism of excision of DNA damage which consists of incision/excision and repair synthesis/ligation. We have adapted the repair synthesis reaction with cells extracts incubated with damaged plasmid DNA performed in liq...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)80038-8
更新日期:1999-01-01 00:00:00
abstract::Living organisms are constantly exposed to oxidative stress from environmental agents and from endogenous metabolic processes. The resulting oxidative modifications occur in proteins, lipids and DNA. Since proteins and lipids are readily degraded and resynthesized, the most significant consequence of the oxidative str...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)80048-0
更新日期:1999-01-01 00:00:00
abstract::pH-Dependence of hydrolytic activity of trypsin has been studied in cationic reverse micellar system of cetyltrimethylammonium bromide (CTAB) in (50% v/v) chloroform/isooctane using a positively charged substrate N(alpha)-benzoyl-L-arginine ethyl ester (BAEE). The pH of the medium was varied from 4.0 to 8.5 with addit...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(99)80007-8
更新日期:1998-12-01 00:00:00
abstract::Natural bovine seminal RNase possesses a potent antitumor action. We have mutagenized monomeric bovine pancreatic RNase A, devoid of any cytotoxic action, to insert residues present at corresponding positions in the subunit of dimeric, antitumor, seminal RNase. Like naturally dimeric seminal RNase, the mutant dimeric ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)88887-2
更新日期:1998-11-01 00:00:00
abstract::Flavodoxins are one domain alpha/beta electron transfer proteins that participate in photosynthetic reactions. All flavodoxins carry a molecule of flavin mononucleotide (FMN), non-covalently bound, that confers redox properties to the protein. There are two structurally distinct flavodoxins, short ones and long flavod...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)88876-8
更新日期:1998-10-01 00:00:00
abstract::The family of interferon regulatory transcription factors (IRF) participates in the virus-induced and dsRNA-stimulated transcriptional regulation of either type I IFN genes or a definite set of genes which can also be activated by IFN. In this review, we place emphasis on the role of IRF-3 that associates with the coa...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(99)80021-2
更新日期:1998-08-01 00:00:00
abstract::We have analyzed changes in the supercoiling of nucleoid DNA of murine fibroblasts cultured in the presence of hydralazine. The entire DNA attached to the nuclear matrix was extracted from the cells and sedimented in neutral sucrose density gradients containing ethidium bromide. Nucleoids from cells treated with hydra...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(98)80025-4
更新日期:1998-07-01 00:00:00
abstract::The automation of the mica surface displacement of a surface force apparatus (SFA) increases the accuracy of the intermolecular force measurements that is obtained without the presence of the experimenter. The automatic device cancels any thermal drift and the stability of the mica surfaces is optimum. The distance be...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)80007-3
更新日期:1998-05-01 00:00:00
abstract::Surfactants carrying either a hydrocarbon or a fluorocarbon alkyl chain have been synthesized. The polar head was either tris(hydroxymethyl)acrylamidomethane (THAM), telomerized THAM, or a glycosylated THAM moiety. The aqueous solubility of some of these molecules was increased by oxidizing to a sulfoxide the thioethe...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(00)80017-6
更新日期:1998-05-01 00:00:00
abstract::Crystals of flavoenzyme L-lactate oxidase from Aerococcus viridans (LOX) have been obtained that diffract to 3.0 A resolution (P2(1)2(1)2(1), a = 118.4 A, b = 138.4 A, c = 194.6 A). Crystallographic studies suggest that the enzyme may exist as an octameric form with non-crystallographic two- and four-fold axes in the ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(98)80072-2
更新日期:1998-04-01 00:00:00
abstract::After the assessment of the antitumor activity of the anthracycline pigments, the S peucetius group of metabolites was discovered and eventually doxorubicin, a major anticancer agent of established clinical usefulness was developed in the early seventies. A second generation of compounds followed, represented mainly b...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(98)80003-5
更新日期:1998-03-01 00:00:00
abstract::The G protein coupled receptors (GPCR) are an important class of proteins that act as signal transducers through the cytoplasmic membrane. Understanding the structure and activation mechanism of these proteins is crucial for understanding many different aspects of cellular signalling. The olfactory receptors correspon...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(98)80019-9
更新日期:1998-02-01 00:00:00
abstract::Conformational flexibility and structural fluctuations play an important role in enzyme activity. A great variety of internal motions ranging over different time scales and of different amplitudes are involved in the catalytic cycle. These different types of motions and their functional consequences are considered in ...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(98)80054-0
更新日期:1998-01-01 00:00:00
abstract::Over the years, imino proton exchange measurements have provided a description of base-pair opening and of properties of internal motions of nucleic acids such as the individual opening of base pairs and the insensitivity of base-pair lifetimes to the nature and stability of neighboring pairs. A recent determination o...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)86936-2
更新日期:1997-12-01 00:00:00
abstract::The Gag-encoded nucleocapsid protein NCp7 (72 amino acids) from HIV-1, the regulatory protein, Vpr (96 amino acids) and numerous derivatives have been synthesized by solid phase method and their structures determined by 2D NMR. In NCp7, the two highly folded zinc fingers of the Cx2Cx4Hx4C type are in close spacial pro...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(97)83501-8
更新日期:1997-11-01 00:00:00
abstract::The eukaryotic genome is compacted in the cell nucleus, in a way that allows its faithful and ordered replication each cell cycle. Chromatin is organized into topologically constrained loops that are anchored to the nuclear matrix by specific attachment regions (SARs). Chromatin loops were proposed to correspond to re...
journal_title:Biochimie
pub_type: 杂志文章,评审
doi:10.1016/s0300-9084(97)82001-9
更新日期:1997-10-01 00:00:00
abstract::Apoptosis is the physiological process by which unwanted cells in an organism are killed. Bcl-2, a membrane-bound cytoplasmic protein, and its close relative Bcl-xL, are both effective inhibitors of apoptosis induced by a wide variety of stimuli in many different cell types. In a previous study, we reported that suppr...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)82011-1
更新日期:1997-10-01 00:00:00
abstract::To elucidate the metabolic function of mRNA polyadenylation in Escherichia coli. we searched for a polyadenylate-binding protein as a potential mediator of the function of the poly(A) moiety. Using a nitrocellulose filter-binding assay and a Northwestern blot technique, a protein in the ribosomal supernatant fraction ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)82741-1
更新日期:1997-09-01 00:00:00
abstract::Labeling of 21-kDa material was observed when bovine brain soluble fraction was incubated with [adenylate-32P]NAD+ in the presence of GTP. The 21-kDa substrate, slightly smaller than C3 substrate in size, was labeled even without C3 exoenzyme. GTP could be replaced by nucleoside triphosphates other than ATP while ATP ...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)86150-0
更新日期:1997-07-01 00:00:00
abstract::In the bacterium Escherichia coli, H-NS-(H1, H1a) is a heat-stable protein with a molecular mass of 15.5 kDa involved in nucleoid organisation and gene regulation linked to certain signal transduction pathways. We have shown that, following addition of preparations of everted inner membrane vesicles, heat-stable cleav...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)80025-9
更新日期:1997-06-01 00:00:00
abstract::The Escherichia coli low molecular mass penicillin-binding proteins (PBP4, PBP5 and PBP6) are a group of penicillin-sensitive enzymes involved in the final stages of cell wall assembly. It has been suggested that these proteins may interact with the periplasmic face of the inner membrane via C-terminal amphiphilic alp...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)83502-x
更新日期:1997-04-01 00:00:00
abstract::The purpose of the present study was to investigate the effect of a concentrated preparation (EPA 30) containing eicosapentaenoic acid (EPA, 20:5 n-3) and docosahexaenoic acid (DHA, 22:6 n-3) on the limiting desaturation steps of the polyunsaturated fatty acid biosynthesis in spontaneously hypertensive rats (SHR). Adu...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)81505-2
更新日期:1997-02-01 00:00:00
abstract::The translation factor EF-P is required for efficient prokaryotic peptide bond synthesis on 70S ribosomes from fMet-tRNAfMet. This protein has been purified from Escherichia coli cells and the gene, efp, encoding it has been cloned and sequenced. We have isolated recombinant clones which overexpress a protein that co-...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)87619-5
更新日期:1997-01-01 00:00:00
abstract::Tri(n-butyl)phosphate (TNBP) and sodium cholate (SC) mixtures have been used to inactivate lipid-enveloped viruses like HIV and hepatitis B. We exploited the use of this combination to purify fibroblast growth factor-2 (FGF-2) from human placenta. Human placentas were extracted in the presence of 0.3% TNBP/0.2% SC and...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)89504-6
更新日期:1996-01-01 00:00:00
abstract::Translational autocontrol of the expression of the ribosomal protein S15 proceeds through the transitory formation of a pseudoknot. A synopsis of the known data is used to propose a molecular model of the mechanism involved and for the role of the pseudoknot. This latter structure is able to recruit 30S ribosomal subu...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(96)80003-4
更新日期:1996-01-01 00:00:00
abstract::The Ty1 retrotransposon of the yeast Saccharomyces cerevisiae is a long terminal repeat mobile genetic element that transposes through an RNA intermediate. Initiation of minus-strand and plus-strand DNA synthesis are two critical steps during reverse transcription of the retrotransposon genome. Initiation of minus-str...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(96)80013-7
更新日期:1996-01-01 00:00:00
abstract::A novel approach to genetic sequence analysis is presented. This approach, based on compression of algorithms, has been launched simultaneously by Grumbach and Tahi, Milosavljevic and Rivals. To reduce the description of an object, a compression algorithm replaces some regularities in the description by special codes....
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)84763-8
更新日期:1996-01-01 00:00:00
abstract::IbpA/B, 16 kDa heat-shock proteins were recently described as recognizing heterologous protein inclusion bodies in Escherichia coli cells; the corresponding genes formed an operon regulated by the rpoH gene product, sigma 32 protein (Burland et al (1993) Genomics 16, 551; Allen et al (1992) J Bacteriol 174, 6938; Chua...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)82643-5
更新日期:1996-01-01 00:00:00
abstract::The kinetics of the native glycogen phosphorylase b from rabbit skeletal muscle and of the enzyme specifically deiminated by peptidylarginine deiminase have been studied. One arginine residue per phosphorylase b monomer is transformed into citrulline after 3 h of incubation with peptidylarginine deiminase. The maximal...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)82188-2
更新日期:1996-01-01 00:00:00
abstract::Acetylcholinesterase (AChE) has been identified and purified from the venom of desert cobra (W aegyptia) to apparent homogeneity using a TSK G 3000 SW gel filtration column and a Mono Q anion-exchange column. AChE was purified to homogeneity as established by sodium dodecylsulfate/polyacrylamide gel electrophoresis. T...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/0300-9084(96)81328-9
更新日期:1996-01-01 00:00:00
abstract::The termination of protein synthesis in bacteria requires codon-specific polypeptide release factors RF-1 (UAG/UAA specific) and RF-2 (UGA/UAA specific). We have proposed that release factors mimic tRNA and recognize the stop codon for polypeptide release (Nakamura et al (1996) Cell 87, 147-150). In contrast to the te...
journal_title:Biochimie
pub_type: 杂志文章
doi:10.1016/s0300-9084(97)86715-6
更新日期:1996-01-01 00:00:00